![]() ![]() |
||||||||||
¡@ |
¦@ ¦P ¬ã ¨s «Ç ¹q ¤l ³ø ²Ä¤»¤Q¤G´Á FEB.10.2019 |
|||||||||
¥»´Á¥Ø¿ý ¦@¦P¬ã¨s«Ç¶Q«»ö¾¹°V½mº[·sª¾½Òµ{ ¤U´Á¥DÃD
¤º¬r¯ÀÀË´ú
§Ö³t³sµ²
¡@ |
¦Û¥Ñ°ò¬O¤@Ó¬ã¨sªº¦ÑijÃD¡AÀô¹Ò¤¤¥R¥¸µÛ¦Û¥Ñ°ò¡C¦ý¦Û¥Ñ°ò«ç»ò²£¥Í«ç»ò®ø¥¢¡H³\¦h¬ã¨s³ø§i±´°Qºç¤¤¶ø¯¦¡A¦ý©¹©¹»¡¤£²M·¡¡AŪªÌ¤]¬Ý¤£©ú¥Õ¡C³o»P¬ã¨s¦Û¥Ñ°òªº¤èªk¦³Ãö¡A¤U±¦V¦U¦ìŪªÌ¤¶²Ð¤@ӦۥѰò°»´ú¤èªk¡A³Q»{¬°¬O¥Ø«e¦Û¥Ñ°òÀË´ú³Ìª½±µ¦³®Ä¥B·s¿oªº¤èªk¡C¤U¤@´Á¹q¤l³ø±N¤¶²Ð¡u¤º¬r¯ÀÀË´ú¡v¡A·q½Ð´Á«Ý¡A¨ÃºÜ¸ÛÅwªï±zq¾\¦@¦P¬ã¨s«Ç¹q¤l³ø¥H¦¬¨ú»ö¾¹¤¶²Ð¡B¬ã¨s·sª¾¡B»P¨C¤ë°V½m½Òµ{¸ê°T¡A§óÅwªï±z»P§ÚÌÁpµ¸¡Aµ¹¤©§ÚÌ«Øij»P¹ªÀy¡C
©ó²Ä61´Á¦@¦P¬ã¨s«Ç¹q¤l³ø¤w¤¶²Ð²ÓMªvÀø¤¤¤ß·sÁʸm¤§¹jÂ÷¾Þ§@½c¡A¥iÀ³¥Î©ó°ª·P¬V©ÊÁ{§É¦^¿é²ÓM¤§³B²z¤Î§K¬ÌÀøªk¤§²ÓM¥Í²£¡A¥ç¥i°õ¦æÀù¯g»²§U²ÓMªvÀø¡A¹jÂ÷¾Þ§@½c©ó108¦~2¤ë18¤é¥¿¦¡¶}©ñ¨Ï¥Î¡AÅwªï°|¤º¦P¤¯¦h¥[§Q¥Î¡A¬ÛÃö¹w¬ù¸ê°T¸Ô¨£Âå¾Ç¬ã¨s³¡ºô¶¡C ¥Î¹q¤l¶¶ºÏ¦@®¶(EPR)´ú¥Íª«©Ê¦Û¥Ñ°ò ½Uß»¬ì§Þ ¥Û®pÃ[ ¥ý¥Í ¡@ ¤°»ò¬O¦Û¥Ñ°ò¡H ¦Û¥Ñ°ò¡]Free Radical¡^¡A¤SºÙ´åÂ÷°ò¡A¬O«ü¤Æ¦Xª«ªº¤À¤l¦b¥ú¼öµ¥¥~¬É±ø¥ó¤U¡A¦@»ùÁäµo¥Í§¡µõ¦Ó§Î¦¨ªº¨ã¦³¤£¦¨¹ï¹q¤lªºì¤l©Î°ò¹Î¡C¦b®Ñ¼g®É¡A¤@¯ë¦bì¤l²Å¸¹©ÎªÌì¤l¹Î²Å¸¹®ÇÃä¥[¤W¤@Ó¡u¡E¡vªí¥Ü¨S¦³¦¨¹ïªº¹q¤l¡C¦p²B¦Û¥Ñ°ò¡]H¡E¡^¡B´â¦Û¥Ñ°ò¡]Cl¡E¡^¡B²B®ñ¦Û¥Ñ°ò(¡EOH)¡A¥Ò°ò¦Û¥Ñ°ò¡]CH3¡E¡^µ¥¡C ¥¿±`ªº¤Æ¾Çª«½è¬O¥Ñì¤lºc¦¨ªº¤À¤l¡A¦P®ÉÄâ±a¦¨¹ï¹q¤l¨Óºû«ù¨ä¤Æ¾Çª¬ºAªº¦w©w¡F¦Ó¦Û¥Ñ°ò§Y¬O§t¦³¤£¦¨¹ï¹q¤lªºì¤l©Î¤À¤l¡A¦]¦¹¥¦·|·m¹Ü¨ä¥¦ì¤l©Î¤À¤lªº¹q¤l¨Ó´ê¹ï¡A¤~¯àÂର¦w©wª¬ºA¡C ®ñ¬O¥Í©R¹B°Ê¹Lµ{¤¤¤£¥i¯Ê¤Öªº¤@ºØ®ðÅé¡A¦Û±q1770 ¦~¥Nªì^°ê¤HJoseph Priestley µo²{®ñ¥H¨Ó, ®ñ¤@ª½³Q¤HÌ»{¬°¬O¤@ºØ¹ï¤HÅé¦Ê¯q¦ÓµL¤@®`ªº®ðÅé¡C ¥i¬O¤µ¤Ñ§Ú̪¾¹D, ¤£ºÞ¬OªÅ®ð¤¤ªº®ñÁÙ¬O¤ô¤¤ªº·»¸Ñ®ñ³£¨ã¦³¸û°ªªº®ñ¤Æ©Ê¡A»PÅK¤@¼Ë¡A³B©óªÅ®ð¤¤ªº¤HÅé¦U³¡¦ì³£·|³Q®ñ¤Æ¦Ó¡u¥Íùסv¡A·íµM³oºØ¥Íù×»PÅK¥Íùפ£¦P¡A¥¦Åé²{¦b¤HÅ骺²ÓM¤W¡A ¯S§O¬O¤HÅ骺¾¹©xÀHµÛ¦~Ä֦ӦѤƧó¬O³oºØ¡u¥Íùסvªºª½Æ[ªí²{¡C ¬¡©Ê®ñª«½è (Reactive oxygen species¡FROS)¬O¤HÅ餺®ñ¤Æ¥NÁ¹Lµ{¤Î¦ÛµM¨¾¿m¨t²Î¤¤¡A©Ò²£¥Í¨ã¦³°ª«×¬¡©Êªºª«½è¡C¬¡©Ê®ñª«½è¤S¤À¬°§t®ñ¦Û¥Ñ°ò¤Î«D¦Û¥Ñ°ò (¹Ï¤@)¡A¦Ó¦Û¥Ñ°ò (Free Radical) ©w¸q¬°¨ã¿W¥ß¤£¦¨¹ï¹q¤lªºì¤l¡B¤À¤l©ÎÂ÷¤l¡A¨Ò¦pH¡E¡B¡EOH ¡BO2 -¡E¡BHO2¡E¡BNO¡BNO2¡A¦Ó§t®ñ¦Û¥Ñ°ò«h©w¸q¬°®ñ®ð¦bÅ餺¥NÁ¤§«á©Òl¥Í¥X¬¡©Ê°ª¡B§ðÀ»©Ê±jªº®ñ®ð¤Æ¦Xª«¡A¦p¡G1O2¡B¡EO2-¡B¡EOH¡BH2O2¡C ¤@¯ë¨Ó»¡¡AROS¥Dn¥i¥H¤À¦¨¥|¤jÃþ¡G (1).¶W®ñ¤Æª«³±Â÷¤l (O2-¡D) (2).²B®ñ¦Û¥Ñ°ò (¡EOH) (3).¹L®ñ¤Æ²B (H2O2) (4).³æ«ºA®ñ (Singlet Oxygen, 1O2)
ROS°£¤FÀô¹Ò¤¤ªºªÅ®ð¦Ã¬V¡BÂ÷¤l¿ç®g¡B©âµÒ©M¤Æ¾Çª«½è³£·|¤Þµo¥H¥~¡F¤HÅ餺¥¿±`¥NÁªº®ñ¤Æ¤ÏÀ³¤]·|²£¥Í¬¡©Ê®ñ¡A²É¸¢Åé¬O¨Å餤¬¡©Ê®ñªº¤@Ó«n¨Ó·½¡A©I§l§@¥Î¹Lµ{¤¤ 98% ªº®ñ®ð·|¦b²ÓM¤º²£¥Í¯à¶q¡A¦ý¬O¬ù 2% ·|¦¨¬°¬¡©Ê®ñ¡A¬¡©Ê®ñ³£¬O¦³®ñ¥NÁªº°Æ²£ª«¡CÅ餺ªº¬¡©Ê®ñ¨Ã¤£Á`¬O¦³®`ªº¡A¥¦¹ï¨Åé¤]¦³¯q³Bªº¡C¨Ò¦p§]¾½²ÓM¦b²ÓM½¤¨ü¨ì¨ë¿E®É¡A·|²£¥Í¤j¶q¬¡©Ê®ñ¨Ó«OÅ@§Ú̪º¨Åé¡AROS ¬O§]¾½²ÓMµo´§§]¾½©M±þ¶Ë§@¥Îªº¥Dn¤¶½è¡C ¾A¶qªº¬¡©Ê®ñ¬Oºû«ù¥¿±`¥Í²z¾÷¯à©Ò¥²»Ý¡A¦p°Ñ»P®ñ¤ÆÁÙìµ¥¥Í¤Æ¤ÏÀ³¡B¶Ð°£¥~¨Ó¯fµß¡B»P°T®§¶Ç»¼µ¥¡F¤ÏÆ[¡A¹L¶qªº¬¡©Ê®ñ«o¬O¨ã¦³¦M®`©Ê¡A¦p¼vÅT¥Í²z«í©w¡B¯}Ãa ¥Íª«¤À¤lµ²ºc¡B¬r±þ¥¿±`²ÓM»P«P¨Ï°ò¦]¬ðÅܵ¥¡A¦]¦¹¡A¦Û¥Ñ°ò¥i¨ã¦³«OÅ@»P¶Ë®`ªºÂù«®ÄÀ³¡C·í¦Û¥Ñ°ò¹L«×²£¥Í®É·|³y¦¨»PÅ餺ªº§Ü®ñ¤Æ¨¾¿m¾÷Âà(Antioxidant Defense Mechanisms)¤§¶¡ªº¤£¥¿Å¡A¶i¦Ó¾ÉP®ñ¤ÆÀ£¤O(Oxidative Stress)ªºµo¥Í¡A³\¦h¬ã¨sÃÒ¾Ú«ü¥X®ñ¤ÆÀ£¤O¹ï²{¥N¤ÆºC©Ê¯fªºµo¥Í¨ã«nªº¨¤¦â¡A¦p®ð³Ý(Asthma)¡BµÇ¯f(Nephropathy)¡B¿}§¿¯f (Diabetes Mellitus)¡BÀù¯g(Cancer)¡B¤ßŦ¦åºÞ¯e¯f(Cardiovascular Diseases)¡B ·R´þ¯f(Acquired Immunodeficiency Syndrome)»P¦Ñ¤Æ(Aging)µ¥¡A¦b¯«¸g¨t²ÎªººC©Ê¯f¡A¦p¤¤·(Stroke)¡B¦Ñ¦~è§b¯g(Alzheimer¡¦s disease)¡B©¬ª÷´Ë¤ó¯g(Parkinson¡¦s disease)¡B¦ë¤B¹y¤ó¯g(Huntington's disease)µ¥¡C ¥Ñ©ó¬¡©Ê®ñ¨ã¦³¸û°ªªº¤ÏÀ³¬¡©Ê¡A¥¦Ì¥i¥H»P³\¦h¤£¦Pªº¤Æ¦Xª«µo¥Í¤Æ¾Ç¤ÏÀ³¡A¥Ñ¦¹²£¥Í¦UºØ¤£¦Pªº¤ÏÀ³¥Í¦¨ª«¡A®Ú¾Ú³o¨Ç¤ÏÀ³¥Í¦¨ª«©ÎªÌ¤ÏÀ³ª«ªºÅܤƵ{«×¥i¥H¶i¦æ©w¶q¤Î©w©Ê¤ÀªR¡C³q±`±Ä¥Îªº¤ÀªR¤èªk¦³¡G¤Æ¾Ç§N¥úªk¡B¿Ã¥ú¥ú«×ªk©M¹q¤l¶¶ºÏ¦@®¶µ¥¤èªk¡C ¤Æ¾Ç§N¥úªk¬O¬¡©Ê®ñÀË´ú¤¤³Ì±`¥Î¨ìªº¹êÅç§Þ³N¡A¨äì²z¬O¤Æ¾Ç§N¥ú¸Õ¾¯»P¬¡©Ê®ñ¤ÏÀ³¥Í¦¨¿EµoºAªº²£ª«¡A²£ª«¤¤ªº¹q¤l¸g«D¿ç®g©ÊÅD¾E¦^¨ì°òºA¦Ó©ñ¥X¥ú¤l¡C±`¥Îªº¤Æ¾Çµo¥ú¸Õ¾¯¦³¾|¦Ì¿Õ(Luminol)¡B¥ú¿Aºë(Lucigenin)©M®ü¿Ã¿Ã¥ú¯À¡]Cypridina Luciferin Analog¡ACLA¡^¬¡©Ê®ñªº¤Æ¾Çµo¥ú¬ã¨s¤]¬O¬¡©Ê®ñ´ú©wªk¬ã¨s»â°ì¤¤¤ñ¸û±`¥Îªº¤èªk¡A¦ý¬O±`¦³±M¤@©Ê¤£¨¬ªº°ÝÃD³y¦¨¬ã¨s¤Hûªº§xÂZ¡C ¹q¤l¶¶ºÏ¦@®¶ªk¡]Electron Paramagnetic Resonance¡^ »P¨ä¥L´ú¸Õ¤èªk¬Û¤ñ¡AEPR¬OÆ[¹î©MÀË´ú¬¡©Ê®ñ³Ìª½±µ©M³Ì¦³®Äªº¤èªk¡CEPR©Ò¨ã¦³ªº¿W¯S©Ê¶V¨Ó¶V¨ü¨ì«µø¡A¦¨¬°ÀË´ú©MÃѧO¬¡©Ê®ñ¤£¥i¯Ê¤Öªº¤u¨ã¡C§@¬°¬¡©Ê®ñªº¤Æ¾Ç¤ÏÀ³«áªºÀË´ú¤èªk¡A¥i¥H²z¸Ñ¦¨¬O¤@ºØ¦Û¥Ñ°òªº¼ÐÃѤÏÀ³, §Y¦V¬¡©Ê®ñ¥Í¦¨ªº¤ÏÀ³Åé¨t¤¤²K¥[¥»¨±a¦³¤£¹ïºÙ¹q¤lªº¦Û¥Ñ°ò, ³o¤@²K¥[ª«»P¤£¦Pªº¬¡©Ê®ñºØÃþµo¥Í¤ÏÀ³¦Ó¾ÉPEPR «H¸¹ªºÅܤƨөw©Ê¬O¨º¤@ºØ¬¡©Ê®ñ¤Î©w¶q¨C¤@ºØ¬¡©Ê®ñªº§t¶q¡C ¹q¤l¶¶ºÏ¦@®¶(Elecron Paramagnetic Resonance, EPR)¡A¤SºÙ¬°¹q¤l¦Û±Û¦@®¶¡]Electron Spin Resonance, ESR¡^¬O°ß¤@¥iÀË´ú§t¦³¥¼¦¨¹ï¹q¤lª«½èªº¥úÃоǧ޳N¡C¦b§Ú̥ͬ¡¤W¸I¨ì¦Û¥Ñ°òªº¾÷·|¬Û·í¦h¡C¦p¹Ï¤G©Ò¥Ü, ¨C¤Ñ©I§lªº®ñ®ð¡B¦¤W°_§É³Üªº©@°Ø¡B¦À\¦Yªº¨©ªG..³o¨Ç§÷®Æ¤º³¡³£¦s¦b¦Û¥Ñ°ò¡C¦Û¥Ñ°òªº¹Ø©R³q±`«Üµu¡A¦ý¥¦Ì¦b¥Í©Rªº¹Lµ{¤¤µo´§µÛ¦ÜÃö«nªº§@¥Î¡A¤ñ¦p¥ú¦X§@¥Î¡B®ñ¤Æ§@¥Î¡B¶Ê¤Æ§@¥Î¡B»E¦X¤ÏÀ³µ¥µ¥¡C ¦]¦¹¡AEPR¬O¤@ºØ¸ó¶V¦hӾǬ쪺§Þ³N¡A¥]¬A¤Æ¾Ç¡Bª«²z¾Ç¡B¥Íª«¾Ç¡B§÷®Æ¬ì¾Ç¡BÂå¾Çµ¥¡C
EPR¥i¥H´£¨Ñþ¨Ç¸ê°T¡H ¥Ø«e¡A¥u¦³EPR§Þ³N¥i¥H©ú½TÀË´ú¥¼¦¨¹ï¹q¤l¡C¿Ã¥úÀË´úµ¥¨ä¥¦§Þ³N¥u¯à´£¨Ñ¦³Ãö¦Û¥Ñ°òªº¶¡±µÃÒ¾Ú¡A¥u¦³EPR¤~¯à½T¤Á¦aÃÒ©ú¦Û¥Ñ°òªº¦s¦b¡C ¦¹¥~¡AEPRÁÙ¾Ö¦³ÀË¥X¨ÃŲ©w¶¶ºÏª«½èªº¿W¯S¯à¤O¡CEPR¼Ë«~¹ï§½³¡Àô¹Ò«D±`±Ó·P¡C ¦]¦¹¡A¸Ó§Þ³N¥i´£¨Ñ¦³Ãö¥¼¦¨¹ï¹q¤lªþªñªº¤À¤lµ²ºcªº¸ê°T¡CEPRÃЦ³®ÉÁÙ·|§e²{¼@¯PªºÃнu§Îª¬ÅܤơA³o¦³§Q©ó¤ÀªR¤Hû²`¤J¬ã¨s¤À¤l¹B°Ê©Î¬y°Ê©Êµ¥°ÊºA¹Lµ{¡CEPR¦Û±Û®·Àò§Þ³N¥iÀË´úµu¹Ø©Rªº¬¡¼â¦Û¥Ñ°ò¡A¬O§Q¥ÎEPR§Þ³NÀË´ú©Mų©w¦Û¥Ñ°òªº¤@Ó½d¨Ò¡C ¸Ó§Þ³N¦b¥Íª«Âå¾Ç»â°ì¤@ª½¥H¨Ó³£¦ÜÃö«n¡A³Q¼sªx¥Î©ó¬ã¨s¦Û¥Ñ°òªº¯f²z¾Ç©M¬r²z¾Ç§@¥Î¡CEPR¦Û±Û¼Ð°O¬O¥Íª«¤Æ¾Ç®a±`¥Îªº¤@¶µ§Þ³N¡A¥¦¨Ï¥Î¶¶ºÏ¤À¤l¡]§Y¦Û±Û¼Ð°O¡^¨Ó¡§¼Ð°O¡¨¯S©w°Ï°ìªº¤j¤À¤l¡C ®Ú¾Ú¦Û±Û¼Ð°Oª«ªºEPRÃСA¥Íª«¤Æ¾Ç®a¥i½T©w¦Û±Û¼Ð°O³B©ó«ç¼ËªºÀô¹Ò¤U¡]²¨¤ô©Ê¡B»ÄÆP«×¡B¬y°Ê©Êµ¥¡^¡C EPRªº¤u§@ì²z
EPR¬O¤@ºØºÏ¦@®¶§Þ³N¡A»P®ÖºÏ¦@®¶¡]NMR¡^«D±`¬Û¦ü¡C
¦ý¬O¡A¸Ó§Þ³N¤£¬O´ú¶q¼Ë«~¤¤ªºì¤l®ÖÅD¾E¡A¦Ó¬OÀË´ú¥¼¦¨¹ï¹q¤l¦b¥~¥[ºÏ³õ¤¤ªºÅD¾E¡C
¹q¤l©M½è¤l¤@¼Ë·|¦³¡§¦Û±Û¡¨¡A©Ò¥H¾Ö¦³¡§ºÏ¯x¡¨³oºØºÏÄÝ©Ê¡C
ºÏ¯x·|¨Ï¹q¤l§Î¦¨Ãþ¦ü¤pºÏ±øªº±Æ¥¬¡A´N¹³±z¶K¦b¦B½c¤W¦B½c¶K¨º¼Ë¡C
·í§Ú̬I¥[¥~³¡ºÏ³õ®É¡A¹q¤l·|¦]¬°¨ä¦Û±Û¦Ó²£¥Í¤è¦V¤£¦PªººÏ¯x»PºÏ³õ¥¦æ©Î¤Ï¥¦æªº¤è¦V±Æ¦C¤À¥¬¡C³o·|¨Ï¹q¤l²£¥Í¨âºØ¯à¶q¤£¦Pªº¯à¶¥¡A¦Ó·í¹q¤l³Q¤À¬°¨âӯඥ®É¡A§ÚÌ«K¥i¥H¥Î·Lªi(Microwave)±N§C¯à¶¥ªº¹q¤l¿Eµo¨ì°ª¯à¶¥¦Ó²£¥ÍEPRªº«H¸¹¡C ¤£¦PªºÀ³¥Î·|¥Î¨ì¤£¦PªºEPR ÀW¹D¡A°ò¥»¤W¦Û¥Ñ°òªºÀ³¥Î³£¬O¨Ï¥ÎX Band¡C
EPR ¥ÑºÏÅK¡B¥D¾÷©M¹q¸£²Õ¦¨¦p¹Ï¤T
¨Ï¥Î¦Û±Û®·®»¾¯¡]Spin Trap¡^ÀË´ú¦Û¥Ñ°ò(ROS) ¹q¤l¶¶ºÏ¦@®¶§Þ³N¡]Electron Paramagnetic Resonance¡AEPR¡^¬O¤@ºØÀË´ú¦Û¥Ñ°òªº¤èªk¡A¥Ñ©óO2-¡P¡B¡P OHµ¥¦Û¥Ñ°òªº¤ÏÀ³¬¡©Ê«Ü°ª¦]¦¹«ÜÃøºû«ùªø®É¶¡ªºÃ©w¦ÓÃø¥HÀË´ú¡AEPR»P¦Û±Û®·®»§Þ³N¡]Spin Trap¡^¬Ûµ²¦X¥i¸Ñ¨M³oÓ°ÝÃD¡Cº¥ý¦Û±Û®·®»¾¯»P¦Û¥Ñ°ò¤ÏÀ³¥Í¦¨¬Û¹ïéwªº¦Û¥Ñ°òªþ¥[¥Í¦¨ª«¡A´N¥i¥H¥ÎEPR¶i¦æ´ú©w¦Û¥Ñ°òªº¤ÀªR¡C ¦Û±Û®·®»¾¯¤@¨Ç§t¦³NO©x¯à°òªº¤Æ¦Xª«³q±`¬Oµvରò(Nitrone)©Î¨Èµv°ò(Nitroso)ªº¤Æ¦Xª«¡A³oÃþ¤Æ¦Xª«¨S¦³¦Û¥Ñ¹q¤l¦]¦¹»P¦Û¥Ñ°ò¤ÏÀ³¦¨¬°ªþ¥[¥Í¦¨ª««eEPR ¬OÀË´ú¤£¨ì¡A¥t¥~³oÃþ¤Æ¦Xª««Üéw¤£®e©ö³Q®ñ¤Æ©ÎÁÙì¡C¦Û±Û®·®»¾¯»P¦Û¥Ñ°ò¤ÏÀ³·|§Î¦¨§téw¦Û¥Ñ¹q¤lªºªþ¥[¥Í¦¨ª«¡A³o¨Çéwªº¤Æ¦Xª«´N¥i¥H³QEPRÀË´ú¡C±`¥Îªº¦Û±Û®·®»¾¯¦pªí¤G¡A EPR ªº¦n³B¬O¤£¦Pªº¦Û¥Ñ°ò»P¦P¤@¦Û±Û®·®»¾¯·|§Î¦¨¤£¦Pªº¯S©Ê§l¦¬¡A¦p¦¹¤@¨Ó§ÚÌ´N¥i¥H¤À¿ë¶W®ñ¤Æª«³±Â÷¤l (O2-¡D)©M²B®ñ¦Û¥Ñ°ò (¡EOH)¡C¦p¹Ï¥|©Ò¥Ü: ¦³¨Ç¦Û±Û®·®»¾¯¹ï²ÓM¨S¦³¬r©Ê¦]¦¹¥i¥HÀË´ú²ÓM¤ºªº¦Û¥Ñ°ò«H¸¹¡A¯à¤À¿ë¤£¦PªºROS¬OEPRªº³Ì¤jÀuÂI¡A¤×¨ä¬O»P¦Û±Û®·®»§Þ³N¬Ûµ²¦X´£°ª¤FÀË´ú¤èªkªºÆF±Ó«×¤Î©w©Ê¤ÀªRªº¥i«H«×¡AÂX¤j¤F¨äÀ³¥Î½d³ò¡C
¹Ï¤©ÒÅã¥Üªº´N¬O¦p¦ó¨Ï¥ÎEPR ¨ÓÀË´úROS
»ö¾¹°V½m½Òµ{:½Òµ{ºô¸ô³ø¦W
¬ã¨s·sª¾½Òµ{:½Òµ{ºô¸ô³ø¦W
Åwªï±zq¾\¦@¦P¬ã¨s«Ç¹q¤l³ø¥H¦¬¨ú»ö¾¹°V½m»P¬ã¨s·sª¾½Òµ{Á¿²ß¬ÛÃö¸ê°T ¡C
¬°«ùÄò´£¨ÑÀu½è¤§¬ã¨sªA°È¡A«K©ó¤é«á¸u¥Î±M¾§Þ³N¤Hû¡BÁʸm·s»ö¾¹¡B²{¦³»ö¾¹¨O´«·s»PºûÅ@«O¾iµ¥µ¥¡A·q½Ð©ó¨Ï¥Î¦@¦P¬ã¨s«Ç¸ê·½¨Ãµoªí½×¤å®É¡A©ó½×¤åPÁ¡]Acknowledgement¡^³B¥[¤JPÁ¦@¦P¬ã¨s«Ç¤§¤å¥y¡A¨Ã©ó½×¤åµoªí®É³qª¾¦@¦P¬ã¨s«ÇºÞ²z¤Hû¡CPÁ¤å¥y½Ð¨Ì¹ê»Ú¨Ï¥Î±¡§Î®Ñ¼g¡A©Î½Ð°Ñ¦Ò¥H¤U½d¨Ò¡GWe
thank the staff of the Core Labs, Department of Medical Research, National
Taiwan University Hospital for technical support. |
|||||||||
²Ä¤»¤Q¤G´Á FEB.10.2019 |